王佳伟-凯发k8

副教授

2001年／中国科学技术大学／学士

2005年／中国科学院物理研究所／博士

2005－2008年／美国阿岗国家实验室／博士后

2008－2011年／清华大学生命科学学院／高级工程师

2011－2017年／清华大学生命科学学院／助理教授

2017年-至今／清华大学生命科学学院／副教授

●  研究兴趣、领域：

本实验室主要利用x射线晶体学和冷冻电镜方法研究细菌磷酸转移酶系统（pts）的膜蛋白结构。其中甘露糖转运复合物（man-pts）是pts的一类重要成员，负责葡萄糖、甘露糖及多种糖类的摄取。此外， man-pts被报道为相关细菌素的作用靶点，其参与或介导了杀菌作用。本课题组已经解析出细菌素作用靶点---大肠杆菌来源的man-pts的结构。后续将继续通过晶体学和冷冻电镜等手段研究细菌素与man-pts作用后的杀菌机制，并为新型抗菌药物和食品添加剂的设计研发提供思路。本课题组同时探索结构解析过程中的困难问题，发展新的结构分析方法。

●  代表性论文：

1. li r, duan j, zhou y, wang j*. structural basis of the mechanisms of action and immunity of lactococcin a, a class iid bacteriocin. appl environ microbiol. 2023; 89:e00066-23.

2. zhu l, zeng j, wang j*. structural basis of the immunity mechanisms of pediocin-like bacteriocins. appl environ microbiol. 2022; 88(13):e0048122.

3. wang c, zeng j*, wang j*. structure basis of bacteriophage lambda capsid maturation. structure, 2022; s0969-2126(21)00461-5.

4. zhu l, zeng j, wang c, wang j*. structural basis of pore formation in the mannose phosphotransferase system by pediocin pa-1. appl environ microbiol. 2022; 88(3):e0199221.

5. huang k, zeng j, liu x, jiang t*, wang j*. structure of the mannose phosphotransferase system (man-pts) complexed with microcin e492, a pore-forming bacteriocin. cell discovery, 2021;7(1):20.

6. li l, dai s, gao gf*, wang j*. lattice-translocation defects in specific crystals of the catalytic head domain of influenza neuraminidase. acta crystallogr d struct biol. 2020;76:1057-1064 (cover).

7. su r*, zhang s, wang j*. automatically generating mask for mapping out the density map in cryoem single-particle reconstruction. progress in biochemistry and biophysics, 2019; 46:1020-1030.

8. liu x, zeng j, huang k, wang j*. structure of the mannose transporter of the bacterial phosphotransferase system. cell research 2019;29(8):680-682.

9. qi x, schmiege p, coutavas e, wang j*, li x*. structures of human patched and its complex with native palmitoylated sonic hedgehog. nature. 2018;7716:128-132.

10. liu x, wang j*, sun l*. structure of the hypersomolality-gated calcium-permeable channel osca1.2. nat commun. 2018; 9(1):5060.

11. zhou n, wang h*, wang j*. embuilder: a template matching-based automatic model-building program for high-resolution cryo-electron microscopy maps. sci rep. 2017;7(1):2664.

12. wang j*, chai j, wang h. structure of the mouse toll-like receptor 13 ectodomain in complex with a conserved sequence from bacterial 23s ribosomal rna. febs j. 2016;283(9):1631-5 (cover).

13. wang j, wang w, song w, han z, zhang h, chai j*, wang h*, wang j*. an improved method for phasing crystal structures with low non-crystallographic symmetry using cryo-electron microscopy data. protein cell. 2015;6(12):919-23.

14. luo p, dai s, zeng j, duan j, shi h, wang j*. inward-facing conformation of l-ascorbate transporter suggests an elevator mechanism. cell discovery, 2018;4:35.

luo p, yu x, wang w, fan s, li x*, wang j*. crystal structure of a phosphorylation-coupled vitamin c transporter. nat struct mol biol. 2015;22(3):238-41 (cover).

●  凯发k8的联系方式：

电话： 86-10-62782124

传真： 86-10-62782124

邮箱：jwwang@tsinghua.edu.cn

通讯地址：清华大学生物医学馆a317室