孙前文-凯发k8

副教授

2000-2004    学士，华中农业大学 生科院

2004-2009    博士，华中农业大学 生科院

2009-2014    博士后，john innes centre, norwich, uk

2014-今 研究员，清华-北大生命科学联合中心

2014-2021   助理教授， 清华大学生命科学学院

2022-今 副教授，清华大学生命科学学院

●  研究兴趣、领域：

我们实验室感兴趣各个层面的基因组调控事件，重点关注r-loop生物学研究与基因组调控。r-loop是由一条单链dna和另一条rna:dna杂合链混合组成的三股染色质结构，在许多生物体中发挥着重要的生物功能。我们以拟南芥和其它模式生物（如大肠杆菌和酵母）为模型来研究r-loop生物学的基本问题，如r-loop形成、稳定与解除的分子机理，以及r-loop如何影响转录调控和基因组维持。

 

●  已发表论文：

(* corresponding or co-corresponding authors; # co-first authors)

1. weifeng zhang, lingling cheng, kuan li, leiming xie, jinyao ji, xue lei, anjie jiang, chunlai chen, haitao li, pilong li and qianwen sun*. evolutional heterochromatin condensation delineates chromocenter formation and retrotransposon silencing in plants. nature plants. 2024. https://www.nature.com/articles/s41477-024-01746-4.

2. weifeng zhang, zhuo yang, wenjie wang and qianwen sun*. primase promotes the competition between transcription and replication on the same template strand resulting in dna damage. nature communications. 2024; 15, 73. https://doi.org/10.1038/s41467-023-44443-0.

3. qin li, jincong zhou, shuai li, weifeng zhang, yingxue du, kuan li, yingxiang wang and qianwen sun*. dna polymerase ε harmonizes topological states and r-loops formation to maintain genome integrity in arabidopsis. nature communications. 2023; 14, 7763. https://doi.org/10.1038/s41467-023-43680-7.

4. jincong zhou, xue lei, sarfraz shafiq, weifeng zhang, qin li, kuan li, jiafu zhu, zhicheng dong, xin-jian he and qianwen sun*. ddm1-mediated r-loop resolution and h2a.z exclusion facilitates heterochromatin formation in arabidopsis. science advances. 2023 aug;9(32):eadg2699. doi: 10.1126/sciadv.adg2699.

5. wei xu#*, chao liu#, zhe zhang#, changbin sun, qin li, kuan li, hui jiang*, wei li* and qianwen sun*. detail-seq is an ultra-efficient and convenient method for meiotic dna break profiling in multiple organisms. sci china life sci, 2023 june; 66(6): 1392–1407. https://doi.org/10.1007/s11427-022-2277-y.

6. kuan li#, zhenzhen wu#, jincong zhou#, wei xu, ling li, chao liu, wei li, chengjun zhang* and qianwen sun*. r-loopatlas: an integrated r-loop resource from 254 plant species sustained by a deep-learning-based tool. molecular plant. 2023 mar;16(3):493-496. doi: 10.1016/j.molp.2022.12.012.

7. liu k and sun q*. intragenic trna-promoted r-loops orchestrate transcription interference for plant oxidative stress responses. the plant cell, 2021, 33: 3574–3591.

8. cheng l#, wang w#, yao y#, sun q*. mitochondrial rnase h1 activity regulates r-loop homeostasis to maintain genome integrity and enable early embryogenesis in arabidopsis. plos biology, 2021, 19(8): e3001357.

9. wang w, li k, yang z, hou q, zhao w w, sun q*. rnase h1c collaborates with ssdna binding proteins why1/3 and recombinase reca1 to fulfill the dna damage repair in arabidopsis chloroplasts. nucleic acids research, 2021 jul; 49(12): 6771–6787.

10. yang z, li m, sun q*. rhon1 co-transcriptionally resolves r-loops for arabidopsis chloroplast genome maintenance. cell reports. 2020 jan; 30(1), 243–256.

11. xu w#, li k#, li s#, hou q#, zhang y, liu k and sun q*. the r-loop atlas of arabidopsis development and responses to environmental stimuli. the plant cell, 2020 apr; 32(4):888-903.

12. zhao s#, cheng l#, gao y#, zhang b#, zheng x, wang l, li p*, sun q*, li h*. plant hp1 protein adcp1 links multivalent h3k9 methylation readout to heterochromatin formation. cell research, 2019 jan; 29(1):54-66.

13. yang z#, hou q#, cheng l#, xu w, hong y, li s, sun q*. rnase h1 cooperates with dna gyrases to restrict r-loops and maintain genome integrity in arabidopsis chloroplasts. the plant cell, 2017 oct; 29(10):2478-2497.

14. xu w, xu h, li k, fan y, liu y, yang x*, sun q*. the r-loop is a common chromatin feature of the arabidopsis genome. nature plants, 2017 sep; 3(9):704-714.

15. sun q, csorba t, skourti-stathaki k, proudfoot nj, dean c*. r-loop stabilization represses antisense transcription at the arabidopsis flc locus. science, 2013 may; 340(6132):619-21.

●  凯发k8的联系方式：

地址：清华大学生物技术馆4段313室(办公室)，309室(实验室)

电话:   86-10-62784002 (办公室); 62796360 (实验室)

电子邮件：sunqianwen(a t)mail.tsinghua.edu.cn